Composite

Part:BBa_K3813001:Design

Designed by: Matthew Kaung   Group: iGEM21_ASIJ_Tokyo   (2021-07-31)


Mucin(MUC 1) with T7 promoter and T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7155
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1905
    Illegal NgoMIV site found at 1965
    Illegal NgoMIV site found at 2024
    Illegal NgoMIV site found at 2079
    Illegal NgoMIV site found at 2114
    Illegal NgoMIV site found at 2174
    Illegal NgoMIV site found at 2354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is part of the 9 constructs we made for optimal production of our biomarker of interest. It was one of the most successful constructs.


Source

The source of the Mucin 1 sequence was obtained from the "Homo sapiens chromosome 1, GRCh38.p13 Primary Assembly" sequence from NCBI.


References